Neonatal macrophages express elevated levels of interleukin-27 that oppose immune responses.

Neonatal macrophages express elevated levels of interleukin-27 that oppose immune responses.

Kraft JD, et al.

Immunology. 2013 Mar 6.

Abstract
Microbial infections are a major cause of infant mortality worldwide as a result of impaired immune defenses in this population. The nature of this work was to further understand the mechanistic limitations of the neonatal and infant immune response. Interleukin (IL)-27 is a heterodimeric cytokine of the IL-12 family that is produced primarily by antigen presenting cells and is immunosuppressive toward a variety of immune cell types. Here we show that IL-27 gene expression is elevated in cord blood-derived macrophages relative to macrophages originating from healthy adults. We also evaluated the duration in which elevated IL-27 gene expression may impact immune responses in mice. Age-dependent analysis of IL-27 gene expression indicated that levels of IL-27 remain significantly elevated throughout infancy and then declined in adult mice. Flow cytometric analysis of intracellular cytokine-stained splenocytes further confirmed these results. IL-27 may be induced during pregnancy to contribute to the immunosuppressive environment at the fetal-maternal interface since we demonstrate dose-responsive gene expression to progesterone in macrophages. Neutralization of IL-27 in neonatal macrophages improved the ability of these cells to limit bacterial replication. Moreover, neutralization of IL-27 during incubation with the Mycobacterium bovis BCG vaccine augmented the level of interferon (IFN)-γ elicited from allogeneic CD4(+) T lymphocytes. This suggests that blocking IL-27 during vaccination and infection may improve immune responses in newborn and infant populations. Furthermore, mice will be a suitable model system to further address these possibilities

Source: Department of Pathology, Microbiology, and Immunology, University of South Carolina School of Medicine, Columbia, South Carolina.

Iklan

Tinggalkan Balasan

Isikan data di bawah atau klik salah satu ikon untuk log in:

Logo WordPress.com

You are commenting using your WordPress.com account. Logout / Ubah )

Gambar Twitter

You are commenting using your Twitter account. Logout / Ubah )

Foto Facebook

You are commenting using your Facebook account. Logout / Ubah )

Foto Google+

You are commenting using your Google+ account. Logout / Ubah )

Connecting to %s